Fig. 5

trans-Resveratrol induces preferential activation of MAP kinases. a MCF-7 cells were treated for 6 h with 50 μg/ml trans-resveratrol; 20 μg of cytosolic extracts were loaded onto each lane for detection of phospho-isoforms of JNK, p38^MAPK and c-Jun. β-actin was used as loading control. Immunoblots are from one experiment representative of three that gave similar results. b MCF-7 cells were incubated with 10 μM SP600125 or SB203580, the specific inhibitors of JNK and p38^MAPK, respectively, for 1 h before and throughout the treatment with 50 μg/ml trans-resveratrol; 20 μg of cytosolic extracts were loaded onto each lane for detection of phospho-c-Jun. β-actin was used as loading control. Immunoblots are from one experiment representative of three that gave similar results. Densitometric analyses of each lane was calculated using Quantity One Software and data are expressed as arbitrary units