Human Parainfluenza Virus Infection of the Airway Epithelium: Viral Hemagglutinin-Neuraminidase Regulates Fusion Protein Activation and Modulates Infectivity

doi:10.1128/JVI.00475-09

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J Virol. 2009 Jul; 83(13): 6900–6908.

Published online 2009 Apr 22. doi: 10.1128/JVI.00475-09

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TABLE 1.

Relevant features of the variants used in experiments

HPIV3 HN HN site Neuraminidase activity (% of wt)^a Receptor avidity (mU NA)
Temp of F triggering (°C)^d
pH 5.0^b pH 7.5^c
wt Not applicable 100 35 40 20
H552Q II 100 55 55 15
T193A I 100 90 90 20
N551D II 100 23 37.5 17.5
^aSee reference 18.

^bDegree of red blood cell (RBC) receptor depletion that results in 50% RBC binding (compared to undepleted RBCs) as an indication of relative avidity. A higher mU value indicates a higher relative avidity (15, 18).

^cSee references 15 and 18.

^dTemperature at which 50% fusion is achieved. This temperature reflects HN's efficiency in triggering F. A lower temperature indicates a higher F-triggering efficiency (18).

HPIV3 HN	HN site	Neuraminidase activity (% of wt)^a	Receptor avidity (mU NA)		Temp of F triggering (°C)^d
HPIV3 HN	HN site	Neuraminidase activity (% of wt)^a	pH 5.0^b	pH 7.5^c	Temp of F triggering (°C)^d
wt	Not applicable	100	35	40	20
H552Q	II	100	55	55	15
T193A	I	100	90	90	20
N551D	II	100	23	37.5	17.5