Figure 3

Effects of inhibitors for MAPK, PI3K, and HO-1 on the suppression of glutamate-induced intracellular ROS production by glyceollins. (A) HT22 cells were treated with various concentrations of glyceollins for 8 h, followed by visualizing cell image under fluorescent microscope after staining with a DCFDA dye to assess intracellular ROS levels (magnification, 400×); (B) Cells were pretreated with inhibitors for 2 h and subsequently incubated in the presence of glutamate and glyceollins for another 8 h. Cell images were visualized by fluorescence microscopy after staining with a DCFDA dye.