FROM:
Mol Cell Biochem 1995 (Jul 19); 148 (2): 183–189
Prasad K, Laxdal VA, Yu M, Raney BL
Department of Physiology,
College of Medicine,
University of Saskatchewan,
Saskatoon, Canada
Garlic has been claimed to be effective against diseases, in the
pathophysiology of which oxygen free radicals (OFRs) have been
implicated. Effectiveness of garlic could be due to its ability
to scavenge OFRs. However, its antioxidant activity is not know.
We investigated the ability of allicin (active ingredient of
garlic) contained in the commercial preparation Garlicin to
scavenge hydroxyl radicals (-OH) using high pressure liquid
chromatographic (HPLC) method. -OH was generated by photolysis of
H2O2
(1.2510 moles/ml) with ultraviolet light and was trapped with
salicylic acid which is hydroxylated to produce OH adduct
products 2,3- and 2,5-dihydroxybenzoic acid (DHBA), H2O2 produced
a concentration-dependent OH as estimated by -OH adduct products
2,3-DHBA and 2,5-DHBA. Allicin equivalent in Garlicin (1.8, 3.6,
7.2, 14.4, 21.6, 28.8 and 36 g) produced concentration-dependent
decreases in the formation of 2,3-DHBA and 2,5-DHBA. The
inhibition of formation of 2,3-DHBA and 2,5-DHBA with 1.8 g/ml
was 32.36% and 43.2% respectively while with 36.0 g/ml the
inhibition was approximately 94.0% and 90.0% respectively. The
decrease in OH adduct products was due to scavenging of OH and
not by scavenging of formed OH adduct products. Allicin prevented
the lipid peroxidation of liver homogenate in a concentration-
dependent manner. These results suggest that allicin scavenges OH
and Garlicin has antioxidant activity.